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Key Documents

A4675

Sigma-Aldrich

Agar

Type E, suitable for plant cell culture

Sinonimo/i:

Agar-agar, Gum agar

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About This Item

Formula condensata:
(C12H18O9)n
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
50405901
eCl@ss:
42040102
NACRES:
NA.21

Origine biologica

algae (Rhodophyceae)

Tipo

Type E

Forma fisica

powder

tecniche

cell culture | plant: suitable

Temp. transizione

transition temp 32-38 °C (1.5%)

applicazioni

agriculture

Temperatura di conservazione

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
GYYDPBCUIJTIBM-DYOGSRDZSA-N

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Descrizione generale

Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked β-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.
Agar or Type E agar is a thermo-reversible, polysaccharide gelling agent. Structurally, it consists of two main fractions about 70% of agarose and 30% of agaropectin. The gelling properties of agar comes from the agarose, the high gel strength backbone consists of a linear polymer of 3-linked ß-D-galactose and 4-linked 3,6-anhydro-α-L-galactose (LA) repeating units.

Applicazioni

Agar has been used as reagent for the following studies:
  • Plant cell culture studies.
  • As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
  • For the solidification of culture medium of cork oak somatic embryos.
  • to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants
Agar suspension culture may be used for the analysis of cell transformation of palyoma virus. It might have been used for incubation of single antibody-forming cell to investigate the plaque formation.
Agar has been used to examine its effect on metal(loid) sensitivity of the Arabidopsis thaliana metal-hypersensitive mutants.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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Melissa S LeBlanc et al.
Journal of biotechnology, 163(1), 1-9 (2012-10-31)
Arsenic is a toxic metalloid and recognized carcinogen. Arsenate and arsenite are the most common arsenic species available for uptake by plants. As an inorganic phosphate (Pi) analog, arsenate is acquired by plant roots through endogenous Pi transport systems. Inside
The structure of agar: Part I. Fractionation of a complex mixture of polysaccharides.
Duckworth M and Yaphe W.
Carbohydrate Research, 16(1), 189-197 (1971)
Selection of Agar Reagents for Medium Solidification Is a Critical Factor for Metal (loid) Sensitivity and Ionomic Profiles of Arabidopsis thaliana
Uraguchi S, et al.
Frontiers in Plant Science, 11, 503-503 (2020)
Human bone marrow colony growth in agar-gel.
B L Pike et al.
Journal of cellular physiology, 76(1), 77-84 (1970-08-01)
AGAR SUSPENSION CULTURE FOR THE SELECTIVE ASSAY OF CELLS TRANSFORMED BY POLYOMA VIRUS.
I MACPHERSON et al.
Virology, 23, 291-294 (1964-06-01)

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