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Key Documents

A7921

Sigma-Aldrich

Agar

suitable for plant cell culture, powder

Sinonimo/i:

Agar-agar, Gum agar

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About This Item

Formula condensata:
(C12H18O9)n
Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
50405901
eCl@ss:
42040102
NACRES:
NA.21

Origine biologica

algae (Rhodophyceae)

Livello qualitativo

Forma fisica

powder

tecniche

cell culture | plant: suitable

Temp. transizione

congealing temperature 30-35 °C

applicazioni

agriculture

Temperatura di conservazione

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
GYYDPBCUIJTIBM-DYOGSRDZSA-N

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Descrizione generale

Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.

Applicazioni

Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
  • of Gamborg′s B5 regeneration medium
  • of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
  • in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings

Azioni biochim/fisiol

Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.

Altre note

Purified for research and protoplast culture.

Nota sulla preparazione

Typical working concentration: 6-12 g/L.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus
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Lippia rotundifolia Cham. is in the family Verbenaceae and is endemic to the Cerrado. This species is aromatic and characterized by the presence of glandular trichomes on its leaves that are rich in monoterpenes. The objective of this study was

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