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Key Documents

A3076

Sigma-Aldrich

Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

clone GE-1, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

mouse

Livello qualitativo

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

secondary antibodies

Clone

GE-1, monoclonal

Forma fisica

buffered aqueous solution

Reattività contro le specie

human

tecniche

direct ELISA: 1:6,000
dot blot: 1:1,000

Isotipo

IgG2b

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

Categorie correlate

Descrizione generale

Binds human IgE; does not bind human IgG, IgA or IgM.
IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.
Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Applicazioni

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :
  • immunoblot assays
  • direct ELISA
  • dot blot
To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Azioni biochim/fisiol

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Stato fisico

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Risultati analitici

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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