86090201
HeLa229 cell line
from human cervix
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About This Item
Codice UNSPSC:
41106514
Prodotti consigliati
Origine biologica
human cervix
Livello qualitativo
Forma fisica
liquid
Modalità di accrescimento
Adherent
Cariotipo
2n = 46
Morfologia
Epithelial
Prodotti
Not specified
Recettori
Not specified
tecniche
cell culture | mammalian: suitable
Malattie correlate
cancer
Condizioni di spedizione
dry ice
Temperatura di conservazione
−196°C
Origine della linea cellulare
Human cervix carcinoma
Descrizione della linea cellulare
Derived from the parent HeLa line but differs chiefly in its relative insusceptibility to polioviruses. The presence of human papilloma virus 18 (HPV-18) sequences in HeLa cells has been reported.
Applicazioni
Tumourigenicity and virus studies, poliovirus 1 and AD3
Profilo DNA
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Terreno di coltura
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Mantenimento delle subcolture
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Altre note
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Esclusione di responsabilità
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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The journal of physical chemistry. A, 119(44), 10854-10862 (2015-10-16)
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C Yee et al.
The American journal of pathology, 119(3), 361-366 (1985-06-01)
A series of human carcinoma cell lines was examined for human papillomavirus (HPV) DNA sequences with the use of HPV-6, HPV-11, HPV-16, and HPV-18 DNA probes. Six of eight cell lines derived from human cervical carcinomas were shown to contain
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