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36798

Sigma-Aldrich

7-(Diethylamino)coumarin-3-carbohydrazide

BioReagent, suitable for fluorescence, ≥95% (HPCE)

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About This Item

Formula empirica (notazione di Hill):
C14H17N3O3
Numero CAS:
100343-98-4
Peso molecolare:
275.30
Beilstein:
5574454
Numero MDL:
MFCD00467583
Codice UNSPSC:
12352125
ID PubChem:
57649662
NACRES:
NA.32

Nome Commerciale

BioReagent

Livello qualitativo

100

Saggio

≥95% (HPCE)

Solubilità

DMF: soluble
acetonitrile: soluble
chloroform: soluble
methanol: soluble

Fluorescenza

λex 450 nm; λem 468 nm in methanol

Compatibilità

suitable for fluorescence

Stringa SMILE

CCN(CC)c1ccc2C=C(C(=O)NN)C(=O)Oc2c1

InChI

1S/C14H17N3O3/c1-3-17(4-2)10-6-5-9-7-11(13(18)16-15)14(19)20-12(9)8-10/h5-8H,3-4,15H2,1-2H3,(H,16,18)
LYBMHAINSFEHRL-UHFFFAOYSA-N

Descrizione generale

7-(Diethylamino)coumarin-3-carbohydrazide is a coumarin-hydrazide reagent typically used as a fluorescent chemical probe for labeling of cellular carbonyls.

Applicazioni

7-(Diethylamino)coumarin-3-carbohydrazide is used for derivatization and detection of carboxylic acid and carbonylated molecules such as lipid peroxidation products (LPP) by electrospray ionization-mass spectrometry (ESI-MS) in positive ion mode. It may be used as a fluorescent label for the determination of lipid aldehydes in food products by normal phase liquid chromatography (NPLC) coupled to ESI-MS and fluorescent detector (FLD) and for the derivatization of monoterpene alcohols in urine prior to analysis by high performance liquid chromatography(HPLC) with FLD coupled to ESI-MS.

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Altre note

Derivatizing agent for carboxylic acid detection

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319,H335

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
BCCH0909
BCCF3603
BCBW6081
BCBV4048
BCBR8854V

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M.L. Grayeski et al.
Analytical Chemistry, 59, 1203-1203 (1987)
Selective labeling for the identification and semi-quantification of lipid aldehydes in food products
Hollebrands B, et al.
Analytical and Bioanalytical Chemistry, 85(1), 1-9 (2018)
Roland J W Meesters et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 875(2), 444-450 (2008-10-11)
A method was developed for the determination of the monoterpene alcohols verbenol, myrtenol, perillyl alcohol, alpha-terpineol, Delta(3)-carene-10-ol, thymol and p-alpha,alpha-trimethylbenzylalcohol in urine samples. After an enzymatic cleavage of their glucuronide- and sulfate conjugates the monoterpene alcohols were converted in the
Ivana Milic et al.
Analytical chemistry, 85(1), 156-162 (2012-11-29)
Reactive oxygen species (ROS) and other oxidative agents such as free radicals can oxidize polyunsaturated fatty acids (PUFA) as well as PUFA in lipids. The oxidation products can undergo consecutive reactions including oxidative cleavages to yield a chemically diverse group
J C Touchstone et al.
Steroids, 56(12), 601-605 (1991-12-01)
7-Diethylaminocourmarin-3-carbohydrazide was used to label the ketone group of the tetrahydroprogesterones to form fluorescent derivatives with high sensitivity. The four isomeric 3-hydroxypregnanes separated readily on high-performance, thin-layer chromatography after derivatization. This separation was not possible with the underivatized isomers. Standards
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