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55155

7-Hydroxycoumarin-3-carboxylic acid

Name: 7-Hydroxycoumarin-3-carboxylic acid
Brand: SIGMA

suitable for fluorescence, ≥98.0% (capillary electrophoresis)

Sinonimo/i:

Umbelliferone-3-carboxylic acid

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About This Item

Formula empirica (notazione di Hill):

C₁₀H₆O₅

Numero CAS:

779-27-1

Peso molecolare:

206.15

Beilstein:

384141

Numero MDL:

MFCD00017491

Codice UNSPSC:

12352106

ID PubChem:

57651515

NACRES:

NA.32

Saggio

≥98.0% (capillary electrophoresis)

Forma fisica

solid

pKa

7.04

Punto di fusione

261 °C (lit.)

Solubilità

DMF: soluble
DMSO: soluble
alcohols: soluble

Fluorescenza

λ_ex 342 nm; λ_em 447 nm (pH 4.0)
λ_ex 386 nm; λ_em 448 nm in 0.1 M Tris pH 9.0

Compatibilità

suitable for fluorescence

Stringa SMILE

OC(=O)C1=Cc2ccc(O)cc2OC1=O

InChI

1S/C10H6O5/c11-6-2-1-5-3-7(9(12)13)10(14)15-8(5)4-6/h1-4,11H,(H,12,13)
LKLWLDOUZJEHDY-UHFFFAOYSA-N

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Applicazioni

7-Hydroxycoumarin-3-carboxylic acid (7-OHCCA) is used as a fluorogenic substrate for glycosyltransferase assays. 7-OHCCA may be used as a non-cinnamate-class substrate analog to study 4-hydroxycinnamate decarboxylase (4-HCD)-inducing activity in Klebsiella oxytoca cells. It is suitable as a pH indicator.

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Altre note

Immobilized on a support to give a pH sensor

Pittogrammi

GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319,H335

Consigli di prudenza

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Photoactive yellow protein-based protein labeling system with turn-on fluorescence intensity.

Yuichiro Hori et al.

Journal of the American Chemical Society, 131(46), 16610-16611 (2009-11-03)

Protein labeling provides significant information about protein function. In this research, we developed a novel protein labeling technique by utilizing photoactive yellow protein (PYP). PYP is a small protein (14 kDa) derived from purple bacteria and binds to 7-hydroxycoumarin-3-carboxylic acid

Induction of 4-hydroxycinnamate decarboxylase in Klebsiella oxytoca cells exposed to substrates and non-substrate 4-hydroxycinnamate analogs.

Y Hashidoko et al.

Bioscience, biotechnology, and biochemistry, 65(12), 2604-2612 (2002-02-06)

The 4-hydroxycinnamate decarboxylase (4-HCD)-inducing activity of several substrate analogs toward Klebsiella oxytoca was investigated. Four E-cinnamate-class compounds, E-4-hydroxycinnamic acid (1), caffeic acid (2), ferulic acid (3) and E-2,4-dihydroxycinnamic acid (4), all of which were accepted as substrates, all of which

H. Offenbacher et al.

Sens. Actuators, 9, 73-73 (1986)

A fluorometric assay for glycosyltransferase activities using sugars aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid as substrates and high performance liquid chromatography.

K Higai et al.

Biological & pharmaceutical bulletin, 22(4), 333-338 (1999-05-18)

We developed a novel fluorometric assay method for the measurement of glycosyltransferase activities using mono- and di-saccharides aminated and tagged with 7-hydroxycoumarin-3-carboxylic acid (coumarin) as substrates, N-acetylglucosamine (GlcNAc)-coumarin for beta1,4-galactosyltransferase from bovine milk and Gal beta1-4GlcNAc-coumarin for alpha2,3- and alpha2,6-sialyltransferases

Identification and quantification of unknown antioxidants in plastic materials by ultrasonic extraction and ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.

Hang Wang et al.

European journal of mass spectrometry (Chichester, England), 22(1), 19-29 (2016-02-11)

Mass spectrometry has been applied to the targeted analysis of commonly used additives (such as Irganox 1010, Irganox 1076, Irgafos 168, etc.) in plastic materials, but a fast and straightforward method for the non-targeted identification and quantification of unusual or

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