30915

Denhardt′s solution

BioReagent, for molecular biology, lyophilized powder

• Numero MDL: MFCD00717549
• Codice UNSPSC: 12352202
• NACRES: NA.52

Proprietà

• Grado: for molecular biology
• Nome Commerciale: BioReagent
• Forma fisica: lyophilized powder
• Impurezze: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• Compatibilità: suitable for nucleic acid hybridization
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Applicazioni

Suitable to block non-specific binding sites in western, northern and Southern blot membranes

Componenti

Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).

Nota sulla preparazione

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)