T15760

Trizma^® cloridrato

reagent grade, ≥99%, crystalline

• Sinonimo/i: TRIS, TRIS HCl, Tris(idrossimetil)amminometano, Trometano cloridrato
• Formula condensata: NH₂C(CH₂OH)₃ · HCl
• Numero CAS: 1185-53-1
• Peso molecolare: 157.60
• Beilstein: 3675235
• Numero MDL: MFCD00012590
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Origine biologica: synthetic
• Livello qualitativo: 200
• Grado: reagent grade
• Saggio: ≥99%
• Forma fisica: crystalline
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: <0.5% water
• Colore: white
• Intervallo di pH utile: 7.0-9.0
• pKa (25 °C): 8.1
• Punto di fusione: ~150.7 °C
• Solubilità: water: 1.5 g/mL, clear, colorless
• Cationi in tracce: Fe: ≤5 ppm; Pb: ≤5 ppm
• Temperatura di conservazione: room temp
• Stringa SMILE: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H; QKNYBSVHEMOAJP-UHFFFAOYSA-N

Descrizione

Descrizione generale

Tris HCl, or Trizma^® hydrochloride, serves as a widely utilized acidic buffer with a pKa of 8.08 at 25°C, offering a pH range of 7.0 to 9.0, aligning with the typical pH of living organisms. It is a crucial component in buffer solutions for diverse biological, cell biology, and biochemistry applications. Notably, Tris base and Tris hydrochloride individually lack sufficient buffering capacity, necessitating their combination to create effective buffers within the pH range of 7 to 9.

Essential in protein electrophoresis and western blotting techniques, Tris buffers, particularly low ionic strength versions, are employed for protein transfer. They are also commonly used in SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, integral to DNA agarose electrophoresis, contribute to buffer preparations like TAE (Tris acetate/EDTA) and TBE (Tris borate/EDTA), playing pivotal roles in biochemistry, protein purification, and electrophoresis applications. Overall, Tris HCl proves to be a versatile and indispensable component, ensuring optimal pH in biological systems for effective biochemical and cell biology research.

Caratteristiche e vantaggi

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Altre note

I valori di pH di tutti i tamponi dipendono dalla temperatura e dalla concentrazione. Per i tamponi Tris, a ogni °C di diminuzione della temperatura corrisponde un aumento di circa 0,03 unità di pH, mentre a ogni diluizione di un fattore 10 corrisponde una diminuzione di 0,03-0,05 unità di pH. Per applicazioni che necessitano di estrema precisione, utilizzare un pH-metro accuratamente calibrato con un elettrodo combinato vetro/calomelano.

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The pH values of all buffers are temperature and concentration-dependent. For Tris buffers, pH increases by about 0.03 unit per °C decrease in temperature and decreases by 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable