PPB010

Tris EDTA Buffer, pH 8.0

pHast Pack^™, powder, 10X concentrate

• Sinonimo/i: 10X TE buffer
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Nome Commerciale: pHast Pack^™
• Forma fisica: powder
• Condizioni di stoccaggio: (Tightly closed. Dry)
• Impurezze: ≤5 ppm Heavy Metals (lead)
• Colore: white
• pH: 7.9—8.1
• Solubilità: soluble, clear, colorless
• Cationi in tracce: Fe: <10 ppm
• Attività estranea: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

10X Tris-EDTA or TE buffer has two components. Tris buffer is a commonly used stable, non-toxic, and non-hygroscopic buffer. Also, ethylenediaminetetraacetic acid (EDTA), a metal chelator, binds with cation metal ions that further inactivate the DNase enzyme which requires metal ions to activate. It has a pH range of 7.9 - 8.1.

Applicazioni

TE buffer is intended for use in:

• Nucleic acid gel electrophoresis
• storage and dilution of DNA and RNA
• DNA automated sequencing
• Immunohistochemistry

Caratteristiche e vantaggi

• Fast and easy Ready-to-use buffer
• Minimizes effort - no need to weigh and adjust pH
• Saves time and eliminates exposure to hazardous chemicals
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Confezionamento

Foil pouch

Ricostituzione

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 10X solution containing 100mM Tris, 10mM EDTA, pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Stoccaggio e stabilità

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Warning
• Indicazioni di pericolo: H373
• Consigli di prudenza: P260 - P314 - P501
• Classi di pericolo: STOT RE 2 Inhalation
• Organi bersaglio: Respiratory Tract
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable