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E7889

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

Sinonimo/i:

(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2

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About This Item

Formula condensata:
[-CH2N(CH2CO2Na)CH2CO2H]2
Numero CAS:
139-33-3
Peso molecolare:
336.21
Beilstein:
3822669
Numero MDL:
MFCD00070672
Codice UNSPSC:
12352107
ID PubChem:
24894660
NACRES:
NA.31

Grado

for molecular biology

Livello qualitativo

300

Sterilità

0.2 μm filtered

Forma fisica

liquid

Impiego in reazioni chimiche

reagent type: chelator

Concentrazione

0.5 M in H2O

pH

7.5-8.5

Compatibilità

suitable for molecular biology

applicazioni

microbiology

Attività estranea

DNase, RNase, NICKase and protease, none detected

Stringa SMILE

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2
ZGTMUACCHSMWAC-UHFFFAOYSA-L

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Descrizione generale

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Applicazioni

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Caratteristiche e vantaggi

molecular biology grade

Nota sulla preparazione

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Altre note

For additional information on our range of Biochemicals, please complete this form.

Prodotto comparabile

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Warning

Indicazioni di pericolo

H373

Consigli di prudenza

P260 - P314 - P501

Classi di pericolo

STOT RE 2 Inhalation

Organi bersaglio

Respiratory Tract

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLCL5409
BCCG6590
BCCG1836
BCCG0945
BCCF9230

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