T6455
Tris-HCl Buffer, pH 10, 10×, Antigen Retriever
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About This Item
Codice UNSPSC:
12352107
NACRES:
NA.47
Prodotti consigliati
Origine biologica
synthetic
Livello qualitativo
pH
10
applicazioni
hematology
histology
Temperatura di conservazione
2-8°C
Applicazioni
Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.
Nota sulla preparazione
Dilute the Tris-HCl Buffer, pH 10, 10×, and Antigen Retriever 10-fold with water to prepare a 1 × Working Solution, e.g., dilute 10 mL of 10 × concentrate with 90 mL of water.
Stoccaggio e stabilità
Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.
Altre note
Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer. Store the prepared 1′ Tris-HCl Buffer, pH 10, Working Solution at 2–8 °C.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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