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Key Documents

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48900

Sigma-Aldrich

Giemsa Stain, Modified Solution

(for the staining (of cellular blood components and blood parasites))

Sinonimo/i:

Azure eosin methylene blue, Giemsa solution

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About This Item

Numero CAS:
51811-82-6
Numero CE:
200-659-6
Numero MDL:
MFCD00081642
Codice UNSPSC:
41116121
ID PubChem:
329757421
NACRES:
NA.25

Tipo

(for research use only)

Livello qualitativo

200

Durata

limited shelf life

tecniche

microbe id | staining: suitable

Compatibilità

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

Stringa SMILE

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
NALREUIWICQLPS-UHFFFAOYSA-N

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Applicazioni

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Avvertenza

store at 20°C

Principio

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Stato fisico

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Ricostituzione

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

Avvertenze

Danger

Indicazioni di pericolo

H225,H301 + H311 + H331,H370

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Organi bersaglio

Eyes

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

53.6 °F

Punto d’infiammabilità (°C)

12 °C

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCG6252
BCCD3234
BCCB7813
BCBV9977
BCBS3921V

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