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114391

Sigma-Aldrich

Bromophenol Blue

ACS reagent

Sinonimo/i:

3′,3″,5′,5″-Tetrabromophenolsulfonphthalein, Bromphenol Blue Sultone Form

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About This Item

Formula empirica (notazione di Hill):
C19H10Br4O5S
Numero CAS:
115-39-9
Peso molecolare:
669.96
Beilstein:
61698
Numero CE:
204-086-2
Numero MDL:
MFCD00005875
Codice UNSPSC:
12171500
ID PubChem:
24847169
NACRES:
NA.47

Grado

ACS reagent

Livello qualitativo

200

Forma fisica

powder

tecniche

titration: suitable

visual transition interval

3.0-4.6, yellow to blue

Punto di fusione

273 °C (lit.)

Solubilità

ethanol: 0.1%

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4

InChI

1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
UDSAIICHUKSCKT-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Applicazioni

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
BCCG4635
BCCG0495
BCCD6533
SHBL2679
SHBL1423

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