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Key Documents

ROAHA

Roche

Anti-HA (12CA5)

from mouse IgG2bκ

Sinonimo/i:

antibody

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About This Item

Codice UNSPSC:
12352203

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

12CA5, monoclonal

Saggio

>90% (HPLC and SDS-PAGE)

Forma fisica

frozen liquid (11666606001)
lyophilized (11583816001)

Confezionamento

pkg of 200 μg (11583816001)
pkg of 5 mg (11666606001 [1 ml])

Produttore/marchio commerciale

Roche

Concentrazione

5 mg/ml (11666606001)

Isotipo

IgG2bκ

Sequenza dell’epitopo

YPYDVPDYA

Temperatura di conservazione

−20°C

Descrizione generale

Hemagglutinin HA is a dominant antigen present on the influenza viral surface. HA is a homotrimer, in which each monomer is produced as a single polypeptide. This monomer is cleaved into two subunits HA1 and HA2 by the host protease. The HA gene is mapped to segment 4 of influenza B viral genome.

Specificità

Anti-HA (12CA5) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Immunogeno

Amino acids 98-106 from the human influenza virus hemagglutinin protein.

Applicazioni

Use Anti-HA (12CA5) for the detection of native influenza hemagglutinin protein and recombinant HA-tagged proteins using:
  • Dot blots
  • Immunochemistry
  • Immunoprecipitation
  • Western blotting
Note: For experiments in which sensitivity is not critical, use Anti-HA (12CA5). For higher sensitivity detection in western blotting at 10-fold lower concentration, use Anti-HA High Affinity (3F10).

Azioni biochim/fisiol

Membrane fusion and receptor-binding action is the key function of hemagglutinin (HA) and it aids in viral entry and infection. HA is an influenza-virus glycoprotein that regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection.

Qualità

Each lot of Anti-HA antibody is tested for immunoreactivity and purity relative to a reference standard.

Nota sulla preparazione

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 0.1 to 1 μg/ml
  • Immunfluorescence: 1-10 μg/ml
  • Western blot: 0.1 to 1 μg/ml

Ricostituzione

11 583 816 001: Add 0.5 ml of PBS to a final concentration of 0.4 mg/ml.

Altre note

For life science research only. Not for use in diagnostic procedures.

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Codice della classe di stoccaggio

13 - Non Combustible Solids

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus.
Stevens J, et al.
Science, 312(5772), 404-410 (2006)
On the origin of the human influenza virus subtypes H2N2 and H3N2
Scholtissek C, et al.
Virology, 87(1), 13-20 (1978)
Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin.
Skehel J J and Wiley D C
Annual Review of Biochemistry, 69(1), 531-569 (2000)
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