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11585762001

Roche

DIG Wash and Block Buffer Set

greener alternative

storage temp.:2-8°C

Sinonimo/i:

wash and block buffer set, dig

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About This Item

Codice UNSPSC:
41105326

Livello qualitativo

100

Produttore/marchio commerciale

Roche

Caratteristiche più verdi

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Categoria alternativa più verde

, Aligned

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included).
Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Applicazioni

The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.

Confezionamento

1 set containing 4 components.

Qualità

The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.

Nota sulla preparazione

Working solution: dATP, dGTP, dTTP mixture:
For one labeling reaction pipette:
1 μl dATP, (vial 2)
1 μl dGTP, (vial 4)
1 μl dTTP, (vial 5)
to a reaction vial.

Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.
Storage conditions (working solution):
  • After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.
  • When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Altre note

For life science research only. Not for use in diagnostic procedures.

Solo come componenti del kit

N° Catalogo
Descrizione
  • Washing Buffer 10x concentrated
  • Maleic acid Buffer 10x concentrated
  • Blocking Solution 10x concentrated
  • Detection Buffer 10x concentrated

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicabledoes not flash

Punto d’infiammabilità (°C)

Not applicabledoes not flash

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