10165875001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

from Leuconostoc mesenteroides

• Sinonimo/i: G6P-DH, Glucose-6-Phosephate Dehydrogenase
• Classificazione EC (Enzyme Commission): 1.1.1.49 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204

Proprietà

• Origine biologica: bacterial (Leuconostoc mesenteroides)
• Livello qualitativo: 100
• Stato: solution; suspension
• Attività specifica: ~550 units/mg protein (At 25 °C (650 U/mg at 30 °C) with glucose-6-P and NAD as the substrates.)
• PM: dimer 110 kDa
• Confezionamento: pkg of 1 mL (1,000 U)
• Produttore/marchio commerciale: Roche
• Concentrazione: ≥0.1-1.0 % (w/w)
• tecniche: activity assay: suitable
• Colore: white
• pH ottimale: 7.0-8.5(maximal activity at 7.8)
• Solubilità: water: miscible
• N° accesso NCBI: AAA25265
• N° accesso UniProt: P11411
• applicazioni: life science and biopharma
• Attività estranea: 6-PGDH <0.001%; CK <0.001%; GR <0.01%; HK <0.05%; NADH oxidase <0.02%; PGI <0.01%
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Glucose-6-Phosphate Dehydrogenase (G6PD) is a ubiquitous enzyme which acts as a catalyst in producing pentose. It also produces NADPH for various biosynthetic and detoxification reactions. Sometimes G6PD provides an alternative to main glycolytic pathway for glucose utilization. It also has its application as DNA markers on X-chromosome.1

Specificità

Specificity: At pH 7.8, 25 °C: G6P-DH from Leuconostoc (LG6PDH) is highly specific for D-glucose-6-phosphate (K_m = 36 μM, NADP as coenzyme; 64 μM, NAD as coenzyme), but will use either NADP (K_m = 7.4 μM; relative rate 1.0) or NAD (K_m = 115 μM; relative rate 1.8) as coenzyme.
LG6P-DH does not react with fructose-6-phosphate, fructose-1,6-biphosphate, glucose-1-phosphate or ribose-1-phosphate. LG6P-DG will oxidize 2-deoxy-glucose-6-phosphate with NADP, but not with NAD as coenzyme. There is a slow reaction with D-glucose.
Heat inactivation: The ammonium sulfate suspension is not inactivated when heated to temperatures ≤ 50 °C for 10 minutes. At temperatures > 60 °C the enzyme is rapidly inactivated.

Applicazioni

Component of cofactor recycling systems for NADPH.

It was used in enzymatic determination of C6 phosphorylation of glycogen.

Qualità

Contaminants: <0.001% CK, <0.01% GR and PGI each, <0.02% “NADH oxidase”, <0.05% HK, <0.001% 6-PGDH

Definizione di unità

Unit Conversion: One unit (U) [+25 °C] ≈ 1.18 U [+30 °C] ≈ 1.45 U [+37 °C], all with NAD as coenzyme

Unit Definition: One unit (U) LG6P-DH oxidizes 1 mol of glucose-6-phosphate and reduces 1 mol of NAD in 1 minute at +25 °C and pH 7.8.

Stato fisico

Solution of 1,000 U in 1 ml 3.2 M ammonium sulfate, pH approximately 6

Nota sulla preparazione

Activator: HCO^3- (≤ 0.3 M) activates slightly.

Risultati analitici

Absorbance of purified enzyme: 1.15 (1 mg enzyme/ml, 280.5 nm)

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash