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G7877

Sigma-Aldrich

Glucosio-6-fosfato deidrogenasi

Type VII, ammonium sulfate suspension, ≥200 units/mg protein

Sinonimo/i:

G-6-P-DH, Zwischenferment

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About This Item

Numero CAS:
9001-40-5
Classificazione EC (Enzyme Commission):
1.1.1.49 (BRENDA, IUBMB)
Numero CE:
232-602-6
Numero MDL:
MFCD00081656
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

bakers yeast

Tipo

Type VII

Forma fisica

ammonium sulfate suspension

Attività specifica

≥200 units/mg protein

PM

128 kDa

tecniche

cell based assay: suitable

Solubilità

water: soluble

applicazioni

diagnostic assay manufacturing

Temperatura di conservazione

2-8°C

Informazioni sul gene

Saccharomyces cerevisiae ... ZWF1(855480)

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Categorie correlate

Descrizione generale

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).

Applicazioni

Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.

Azioni biochim/fisiol

La glucosio-6-fosfato deidrogenasi catalizza la conversione del glucosio-6-fosfato in 6-fosfoglucono-acetone come prima fase della via dei pentoso fosfati.

Definizione di unità

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

Stato fisico

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 7.0

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Xuehuai Shen et al.
General and comparative endocrinology, 177(1), 177-186 (2012-04-03)
Dehydroepiandrosterone (DHEA), a major steroid hormone, decreases with age, and this reduction has been shown to be associated with physical health. In the present study, the effect of DHEA on cell growth and mitochondrial function was investigated using TM-3 cells
D Janowski et al.
Theriogenology, 78(3), 656-669 (2012-05-15)
The close contact and interaction between the oocyte and the follicular environment influence the establishment of oocyte developmental competence. Moreover, it is assumed that apoptosis in the follicular cells has a beneficial influence on the developmental competence of oocytes. The
Gang Zhao et al.
Neurochemistry international, 61(2), 146-155 (2012-05-15)
NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD
Johannes Yayo et al.
Applied and environmental microbiology, 87(9) (2021-02-21)
The native ability of Clostridium thermocellum to efficiently solubilize cellulose makes it an interesting platform for sustainable biofuel production through consolidated bioprocessing. Together with other improvements, industrial implementation of C. thermocellum, as well as fundamental studies into its metabolism, would
Nanette G Vrijenhoek et al.
ALTEX, 39(2), 207–220-207–220 (2022-01-19)
Chemical read-across is commonly evaluated without specific knowledge of the biological mechanisms leading to observed adverse outcomes in vivo. Integrating data that indicate shared modes of action in humans will strengthen read-across cases. Here we studied transcriptomic responses of primary
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Articoli

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolli

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

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