10109886001
Roche
Trypsin Inhibitor
from soybean
Sinonimo/i:
trypsin
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About This Item
Codice UNSPSC:
12352207
Prodotti consigliati
Origine biologica
soybean
Livello qualitativo
Stato
lyophilized
PM
Mr 20,100
Confezionamento
pkg of 50 mg
Produttore/marchio commerciale
Roche
Concentrazione
1 mg/mL
tecniche
cell culture | mammalian: suitable
pH
3.8-4.5
Solubilità
water: soluble
Temperatura di conservazione
2-8°C
Descrizione generale
Soybean trypsin inhibitor (STI) comprises 181 amino acids. It is a monomeric protein corresponding to a molecular weight of 20 kDa and has two disulfide bridges. It belongs to serpins − serine protease inhibitors family.
Specificità
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. It inhibits trypsin, factor Xa, plasmin, and plasma kallikrein activity in serum-free cell culture media. It does not inhibit metallo-, cysteine, aspartic proteases, or tissue kallikrein (serine proteases).
Applicazioni
Trypsin Inhibitor from soybean is used to terminate tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- in the buffer for hepatocyte isolation
- to inhibit trypsin in Corneal epithelial cells
- to inhibit papain activity while treating hippocampal neural stem cells (NSCs)
Azioni biochim/fisiol
Soybean trypsin inhibitor (STI) inhibits myc proto-oncogene protein and is anticarcinogenic. It favors anti-aggregation of platelets and may be a potential protease inhibiting drug. STI inhibits renin and angiotensin.
Soybean trypsin inhibitor is an inhibitor for trypsin, plasmin, and plasma kallikrein. The product is sensitive to heat, alkaline pH, and protein-precipitating compounds.
Specifiche
Inhibiting activity: approximately 70 trypsin inhibitor units/mg lyophilizate at +25°C with benzoyl-L-arginine ethylester as a substrate (approximately 190 trypsin inhibitor units/mg at +25°C with Chromozym TRY as substrate)
Isoelectric point: 4.5
Isoelectric point: 4.5
Definizione di unità
1 U = 270 S & T-units or 90 USP-units
Nota sulla preparazione
Working concentration: 10 to 100 μg/ml
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Working solution: Recommended solvent is distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
The enzyme is stable for 6 months, frozen in aliquots.
Ricostituzione
Sensitive to heat, alkaline pH, and protein-precipitating compounds.
Stoccaggio e stabilità
Store at 2 to 8 °C. (Store dry!)
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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