10109878001
Roche
Trypsin Inhibitor
from chicken egg white
Sinonimo/i:
trypisin
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About This Item
Codice UNSPSC:
12352207
Prodotti consigliati
Origine biologica
chicken egg white
Livello qualitativo
Forma fisica
powder
Confezionamento
pkg of 1 g
Produttore/marchio commerciale
Roche
Concentrazione
1 mg/mL
tecniche
cell culture | mammalian: suitable
pH ottimale
3.8-4.5
Solubilità
water: soluble
Temperatura di conservazione
2-8°C
Descrizione generale
Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).
Applicazioni
Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
- as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
- to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
- to neutralize trypsin post dissociation and trypsinization of brain tissues
- termination of tissue disaggregation and for subcultivation procedures
Nota sulla preparazione
Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.
Ricostituzione
Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.
Stoccaggio e stabilità
Store at 2 to 8 °C. (Store dry!)
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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