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Key Documents

MABC1176

Sigma-Aldrich

Anti-Bax Antibody, clone 6A7

from mouse

Sinonimo/i:

Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

monoclonal

Reattività contro le specie

human

Reattività contro le specie (prevista in base all’omologia)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology)

Confezionamento

antibody small pack of 25 μg

tecniche

immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG1

N° accesso UniProt

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... BAX(581)

Descrizione generale

Apoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax.

Specificità

Clone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region.

Immunogeno

KLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX.

Applicazioni

Detect using this mouse monoclonal Anti-Bax, clone 6A7 Antibody, Cat. No. MABC1176-25UG, validated for use in Immunoprecipitation, Immunofluorescence and Western Blotting.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).

Qualità

Evaluated by Immunoprecipitation in THP-1 cell lysates.

Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.

Stato fisico

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Determinazione del prezzo

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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