MAB4625

Anti-Bcl-X Antibody, clone 2H12

clone 2H12, Chemicon^®, from mouse

• Sinonimo/i: Bcl-2-like Protein
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 2H12, monoclonal
• Reattività contro le specie: rat, pig, mouse, human
• Produttore/marchio commerciale: Chemicon^®
• tecniche: flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG2a
• N° accesso NCBI: NM_001191.2; NM_138578.1
• N° accesso UniProt: Q07817
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... BCL2L1(598)

Descrizione

Descrizione generale

Bcl-X has two isoforms, Bcl-XL (long), a 241-aa protein, and Bcl-XS (short), a 178-aa protein lacking a 63 aa domain that is well conserved among members of the Bcl-2 family.

Specificità

MAB4625 recognizes a protein of 27 kDa, identified as the Bcl-X protein. Reacts with both Bcl-XS and Bcl-XL.

Immunogeno

Synthetic peptide, aa 3-14 of human Bcl-X protein. This amino acid sequence is shared by human and murine Bcl-X protein.

Applicazioni

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

This Anti-Bcl-X Antibody, clone 2H12 is validated for use in FC, IF, IP, WB, IH for the detection of Bcl-X.

Wstern Blot: 1μg/mL (Hsu, 1997a; Hsu, 1997b)

Flow Cytometry

Immunofluorescence Immunoprecipitation: native protein (2ug/mg protein lysate).

Immunohistochemistry (frozen and paraffin): 2-4ug/mL for 30 minutes at room temperature.

Optimal working dilutions must be determined by end user.

Stato fisico

Format: Purified

Protein A purified immunoglobulin in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Stoccaggio e stabilità

Maintain at 2-8°C for up to 12 months.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable