MAB1614
Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2
clone H2, Chemicon®, from mouse
Sinonimo/i:
Anti-HEL-S-61, Anti-KINH, Anti-KNS, Anti-KNS1, Anti-UKHC
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
H2, monoclonal
Reattività contro le specie
sea urchin, rat, chicken, squid, human, mouse
Reattività contro le specie (prevista in base all’omologia)
mammals
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
radioimmunoassay: suitable
western blot: suitable
Isotipo
IgG2b
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... KIF5B(3799)
Descrizione generale
Kinesins are molecular motors used for intracellular transport and trafficking. They move along microtubule cables powered by the hydrolysis of ATP, thus kinesins are ATPases. The active movement of kinesins supports several cellular functions including mitosis, meiosis and transport of cargo such as axonal transport.
Specificità
Reacts with the heavy chain of kinesin near the N-terminus.
Immunogeno
Bovine brain kinesin.
Epitope: a.a.420-445
Applicazioni
Detect Kinesin using this Anti-Kinesin Antibody, heavy chain, a.a.420-445, clone H2 validated for use in ELISA, IC, IH, IP, FUNC, RIA & WB.
Immunohistochemistry:
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.
Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.
EIA/RIA:
A previous lot of this antibody was used in RIA.
Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.
Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.
Optimal working dilutions must be determined by the end user.
A previous lot was used on paraformaldehyde fixed tissue. Also works for EM.
Immunocytochemistry:
A previous lot of this antibody was used in Immunocytochemistry.
Immunoprecipitation:
A previous lot of this antibody was used in Immunoprecipitation.
EIA/RIA:
A previous lot of this antibody was used in RIA.
Immunoblotting: recognizes 124kDa KHC and its proteolytic fragments.
Inhibition of kinesin mediated transport {Brady, et al PNAS 87(3):1061-1065, 1990.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Qualità
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:500 dilution of this lot detected KINESIN (HEAVY CHAIN) on 10 μg of Mouse Brain lysates.
Descrizione del bersaglio
124 kDa
Stato fisico
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.
Stoccaggio e stabilità
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Risultati analitici
Control
Brain tissue
Brain tissue
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
A monoclonal antibody against kinesin inhibits both anterograde and retrograde fast axonal transport in squid axoplasm.
Brady, S T, et al.
Proceedings of the National Academy of Sciences of the USA, 87, 1061-1065 (1990)
Conventional kinesin holoenzymes are composed of heavy and light chain homodimers.
DeBoer, Scott R, et al.
Biochemistry, 47, 4535-4543 (2008)
T Lynne Blasius et al.
PloS one, 8(9), e76081-e76081 (2013-10-08)
Kinesin motors drive the long-distance anterograde transport of cellular components along microtubule tracks. Kinesin-dependent transport plays a critical role in neurogenesis and neuronal function due to the large distance separating the soma and nerve terminal. The fate of kinesin motors
c-Jun NH2-terminal kinase-interacting protein-3 facilitates phosphorylation and controls localization of amyloid-beta precursor protein.
Muresan, Z; Muresan, V
The Journal of Neuroscience null
Processive bidirectional motion of dynein-dynactin complexes in vitro.
Jennifer L Ross, Karen Wallace, Henry Shuman, Yale E Goldman, Erika L F Holzbaur
Nature Cell Biology null
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