ABT1366

Anti-KIF18A

• Sinonimo/i: Kinesin-like protein KIF18A, MS-KIF18A, Marrow stromal KIF18A
• Codice UNSPSC: 12352203

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• PM: calculated mol wt 102.28 kDa; observed mol wt ~110 kDa
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 100
• tecniche: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG
• Sequenza dell’epitopo: C-terminal
• N° accesso ID proteina: NP_112494
• N° accesso UniProt: Q8NI77
• Temperatura di conservazione: 2-8°C
• Informazioni sul gene: human ... KIF18A(81930)

Descrizione

Specificità

This rabbit polyclonal antibody specifically detects Kinesin-like protein KIF18A. It targets an epitope within 20 amino acids from the C-terminal region.

Immunogeno

KLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Kinesin-like protein KIF18A..

Applicazioni

Quality Control Testing

Evaluated by Western Blotting in HEK293T cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KIF18A in HEK293T cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Human KIF18A in human colon tissue sections.

Immunofluorescence Analysis: A representative lot detected Human KIF18A in Immunofluorescence applications.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrizione del bersaglio

Kinesin-like protein KIF18A (UniProt: Q8NI77; also known as Marrow stromal KIF18A, MS-KIF18A) is encoded by the KIF18A (also known as OK/SW-cl.108) gene (Gene ID: 81930) in human. KIF18A is a widely expressed member of the kinesin superfamily of microtubule-associated molecular motors that hydrolyze ATP to produce force and movement along microtubules. Its kinesin motor domain is localized in amino acids 11-355. It is essential in aligning mitotic chromosomes at the spindle equator during cell division. It plays a role in chromosome congression by reducing the amplitude of pre-anaphase oscillations and slows poleward movement during anaphase, leading to suppression of chromosome movements. It preferentially concentrates on microtubules (MT) plus ends attached to a lagging kinetochore. It possesses both plus-end-directed motility and microtubule depolymerizing activity. It is reported to depolymerize longer microtubules more quickly than shorter ones. The depletion of KIF18A is reported to induce aberrantly long mitotic spindles and loss of tension across sister kinetochores, which results in the activation of the Mad2-dependent spindle-assembly checkpoint. Overexpression of KIF18A is reported in several cancers and is linked to poor overall survival. It is shown to accelerate the invasion and migration of head and neck squamous cell carcinoma. (Ref.: Liu, T., et al. (2023). Biomolecules. 13(2); 326; Liu, G., et al. (2019). Transl. Cancer Res. 8(6); 2252-2263; Du, Y., et al. (2010). Current Biology 20(4); 374-380).

Stato fisico

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Ricostituzione

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stoccaggio e stabilità

Recommended storage: +2°C to +8°C.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.