AB3256-I

Anti-Luciferase

from rabbit, purified by affinity chromatography

• Sinonimo/i: Luciferin 4-monooxygenase
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie (prevista in base all’omologia): all
• Confezionamento: antibody small pack of 25 μL
• tecniche: immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG
• N° accesso UniProt: P08659
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: Photinus pyralis ... Loc116160065(116160065)

Descrizione

Descrizione generale

Luciferin 4-monooxygenase (UniProt: P08659; also known as EC: 1.13.12.7, Luciferase) is a peroxisomal enzyme that belongs to the ATP-dependent AMP-binding enzyme family. It catalyzes the production of oxyluciferin from luciferin in an ATP and magnesium dependent reaction and releases a photon of light when oxyluciferin returns to the ground state. This reaction proceeds in two steps where the first step involves adenylation of luciferin and in the second step luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide, the decay of oxyluciferin producing a photon of light of 562 nm. Luciferase from the firefly is commonly used in reporter assay where the total light emission is proportional to the luciferase activity of the sample.

Specificità

This rabbit polyclonal antibody detects firefly luciferase.

Immunogeno

Epitope: unknown

GST-tagged full length recombinant firefly luciferase.

Applicazioni

Anti-Luciferase, Cat. No. AB3256-I, is a rabbit polyclonal antibody that detects firefly luciferase and has been tested for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Luciferase in HeLa cells transfected with Luciferase expression plasmid.

Research Category
Secondary & Control Antibodies

Qualità

Evaluated by Western Blotting in lysate from HeLa cells transfected with Luciferase expression plasmid.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Luciferase in HeLa cells transfected with luciferase expression plasmid.

Descrizione del bersaglio

~58 kDa observed; 60.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable