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L9420

Sigma-Aldrich

Luciferase from Photinus pyralis (firefly)

recombinant, expressed in E. coli, buffered aqueous solution, ≥10×1010 units/mg protein

Sinonimo/i:

Luciferase firefly

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About This Item

Numero CAS:
61970-00-1
Classificazione EC (Enzyme Commission):
1.13.12.7 (BRENDA, IUBMB)
Numero MDL:
MFCD00131546
Codice UNSPSC:
12352204
NACRES:
NA.54

Ricombinante

expressed in E. coli

Livello qualitativo

200

Saggio

≥98% (SDS-PAGE)

Stato

buffered aqueous solution

Attività specifica

≥10×1010 units/mg protein

PM

62 kDa

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Descrizione generale

Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP.

Applicazioni

Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway.

Definizione di unità

One luciferase enzyme unit will produce one Relative Light Unit (RLU) at 20-25 °C over a 10 second period, measured in 100 μl assay mixture containing 40 pmol ATP and 15 nmol luciferin in Tris-glycine buffer, pH 7.6, using a GloMax 20/20 Luminometer.

Unit Definition Conversion Factor: There are approximately 9000 Relative Light Units (RLU) per one traditional Light Unit that uses a peak height equivalent to 0.02 μCi of 14C in a PPO/POPOP cocktail.

Stato fisico

Supplied a a solution in 25mM Tris-acetate, pH 7.8 , 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol and 30% ethylene glycol

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H302,H334,H373

Classi di pericolo

Acute Tox. 4 Oral - Resp. Sens. 1 - STOT RE 2 Oral

Organi bersaglio

Kidney

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

231.8 °F

Punto d’infiammabilità (°C)

111 °C

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Certificati d'analisi (COA)

Lot/Batch Number
11190522
128M4087V
11180738
058M4116V
11171216

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Sigma-Aldrich

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Griffiths A J F, et al.
An Introduction to Genetic Analysis. (2000)
Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes.
Conti E, et al.
Structure, 4(3), 287-298 (1996)
Reporter gene-facilitated detection of compounds in Arabidopsis leaf extracts that activate the Karrikin signaling pathway.
Sun Y K, et al.
Frontiers in Plant Science, 7, 1799-1799 (2016)
Greta Forlani et al.
Methods in molecular biology (Clifton, N.J.), 1087, 299-313 (2013-10-26)
Human T cell Lymphotropic Viruses 1 and 2 (HTLV-1 and HTLV-2) are the first described human retroviruses. HTLV-1 is the causative agent of an aggressive malignancy of CD4+ T lymphocytes named adult T-cell leukemia/lymphoma (ATLL) and of a chronic neurological
Junji Chida et al.
Methods in molecular biology (Clifton, N.J.), 1098, 21-32 (2013-10-30)
Adenosine 5'-triphosphate (ATP) is the "energy currency" of organisms and plays central roles in bioenergetics, whereby its level is used to evaluate cell viability, proliferation, death, and energy transmission. In this chapter, we describe an improved and efficient method for
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