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Sigma-Aldrich

Rosetta Competent Cells - Novagen

Escherichia coli, rod shaped

Sinonimo/i:

Competent cells

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About This Item

Codice UNSPSC:
41106202
NACRES:
NA.81

product name

Rosetta Competent Cells - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Origine biologica

Escherichia coli

Livello qualitativo

100

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze

Modalità di accrescimento

adherent or suspension

Morfologia

rod shaped

tecniche

microbiological culture: suitable

Trasformazione cellulare

transformation efficiency: >2×106 cfu/μg

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Categorie correlate

Descrizione generale

Genotype: F-ompT hsdSB(rB- mB-) gal dcm pRARE (CamR)





This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Componenti

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Attenzione

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Certificati d'analisi (COA)

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Bekir Altas et al.
Methods in molecular biology (Clifton, N.J.), 1538, 69-82 (2016-12-13)
The chemical synapse displays specialized intercellular adhesion between pre- and potsynaptic plasma membranes mediated by synaptic cell adhesion proteins. In this asymmetric cell adhesion, pre- and postsynapses have their own unique functions; the presynaptic terminal releases neurotransmitter, which diffuses through
Erdem Sendinc et al.
Molecular cell, 75(3), 620-630 (2019-07-08)
mRNA modifications play important roles in regulating gene expression. One of the most abundant mRNA modifications is N6,2-O-dimethyladenosine (m6Am). Here, we demonstrate that m6Am is an evolutionarily conserved mRNA modification mediated by the Phosphorylated CTD Interacting Factor 1 (PCIF1), which
Zsofia Botyanszki et al.
Biotechnology and bioengineering, 112(10), 2016-2024 (2015-05-08)
Biocatalytic transformations generally rely on purified enzymes or whole cells to perform complex transformations that are used on industrial scale for chemical, drug, and biofuel synthesis, pesticide decontamination, and water purification. However, both of these systems have inherent disadvantages related

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