70954
Rosetta(DE3) Competent Cells - Novagen
Escherichia coli, rod shaped
Sinonimo/i:
BL21 derivatives
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About This Item
Codice UNSPSC:
41106202
NACRES:
NA.81
Prodotti consigliati
product name
Rosetta(DE3) Competent Cells - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
Origine biologica
Escherichia coli
Livello qualitativo
Produttore/marchio commerciale
Novagen®
Condizioni di stoccaggio
OK to freeze
Modalità di accrescimento
adherent or suspension
Morfologia
rod shaped
tecniche
microbiological culture: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
−70°C
Categorie correlate
Descrizione generale
Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLysS, the rare tRNA genes are present on the same plasmids that carries the T7 lysozyme gene.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
Componenti
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlRosetta(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
•2 × 0.2 ml5 × 0.2 mlRosetta(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Attenzione
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Note legali
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
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