234138

Human Collagen Type I

from human skin, powder

• Sinonimo/i: Collagen, Type I, Human Skin
• Numero CAS: 9007-34-5
• Numero MDL: MFCD00003075
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Collagen, Type I, Human Skin,
• Origine biologica: human skin
• Livello qualitativo: 100
• Saggio: ≥95% (SDS-PAGE)
• Stato: lyophilized
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• dilution: (compatible for the preparation of collagen gels)
• Impurezze: ≤0.1% subdermal hair
• Solubilità: acetic acid: 5 mg/mL (0.5M)
• Condizioni di spedizione: ambient
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Collagen type 1 is a fibrillar collagen present abundantly in skin, bone, vasculature, and calcified tissues of teeth and bone. It is also seen in organs like the heart and lungs. Collagen type 1 is a heterotrimer consisting of two α1 (CIα1) and one α2 (CIα2) chains with a helical region made of repeating units of proline and hydroxyproline.

Applicazioni

Collagen Type I, Human Skin has been used as a skin protein to study the effect of age-related NADH oxidase (ar-NOX)-catalyzed oxidation on skin proteins. It has also been used as a control sample in High-resolution mass spectrometry (HRMS) analysis.

Azioni biochim/fisiol

Collagen type 1 is a main component of the dermal extracellular matrix (ECM) and participates in skin elasticity. Mutations in collagen type 1 are associated with osteogenesis imperfecta and Ehlers–Danlos syndrome.

Attenzione

Toxicity: Standard Handling (A)

Stato fisico

Salt-free.

Nota sulla preparazione

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Ricostituzione

The recommended concentration is 0.01 to 0.5 M Acetic Acid (or another acidic buffer), with a maximum concentration of 5 mg/ml. The product has been tested at 0.5 M Acetic Acid and is stable for 1 month at 2-8°C. After reconstitution, aliquot and freeze at -20°C. Stock solutions remain stable for up to 6 months at this temperature.

Altre note

Gallop, P.M., and Siefert, S. 1963. Methods Enzymol. 6, 635.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable