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Key Documents

C2249

Sigma-Aldrich

Human Collagen Type I

from human fibroblasts, liquid, 3 mg/mL, suitable for cell culture

Sinonimo/i:

Collagen Solution, Fibroblast Collagen

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.75

product name

Collagen Solution from human fibroblasts, 3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture

Origine biologica

human fibroblasts

Livello qualitativo

Sterilità

sterile-filtered

Nome Commerciale

BioReagent

Forma fisica

solution

Confezionamento

pkg of 20 mL

Concentrazione

3 mg/mL

tecniche

cell culture | mammalian: suitable

Copertura della superficie

6‑10 μg/cm2

Specificità del legame

Peptide Source: Collagen

Peptide Source: Elastin

Attività estranea

endotoxin ≤5.0 μmole/min-mg protein

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Descrizione generale

Collagen constitutes to one-third of the total protein in humans. Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix.Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. It differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Type I collagen is a heterotrimer composed of two α1(I) chains and one alpha2(I) chain, which spontaneously form a triple helix scaffold at neutral pH and 37 °C. Purified human collagen solution is produced from neo-natal human fibroblasts that have secreted human extracellular matrix (hECM) using a propriety cell culture production system. This product contains a high monomer content. In vitro cultures were prepared using intensively tested human fibroblast cells and purified using a manufacturing process following applicable aspects of cGMP. The human fibroblast cells have undergone significant testing demonstrating safety from viruses and other adventitious agents. This process contains built-in, validated steps to ensure the inactivation of possible prion and/or viral contaminants.

Applicazioni

Collagen Solution from human fibroblasts has been used

  • as a component of coating solution for human bone marrow chip fabrication.
  • in fibroblast-like synoviocytes culturing.
  • as a coating solution for microchannels for organ chip culture

Azioni biochim/fisiol

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta. Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.
Proteins belonging to the collagen family supports and nourishes body tissues such as tendons, cartilage, skin and sclera of the eye. Collagen type 1 is a versatile building material required for tissue elasticity and maintains its stability and strength. Collagen is also present in byssus thread of invertebrates, which helps in surface attachment. Defective collagen affects the biomechanical property of arterial tissues.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Collagen: structure and mechanics, an introduction
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Characterize biomarkers measuring extracellular matrix turnover of inflamed osteoarthritis synovium. Human primary fibroblast-like synoviocytes and synovial membrane explants (SMEs) treated with various cytokines and growth factors were assessed by C1M, C3M, and acMMP3 in the conditioned medium. TNFα significantly increased
Aspek Klinik, Genetik dan Molekuler Osteogensis Imperfekta
Mariska M and Auerkari EI
Journal of Dentistry Indonesia, 14(2), 95-110 (2015)
Frederick Grinnell
Trends in cell biology, 13(5), 264-269 (2003-05-14)
Research on fibroblast biology in three-dimensional collagen matrices offers new opportunities to understand the reciprocal and adaptive interactions that occur between cells and surrounding matrix in a tissue-like environment. Such interactions are integral to the regulation of connective tissue morphogenesis
Synovitis biomarkers: ex vivo characterization of three biomarkers for identification of inflammatory osteoarthritis
Kjelgaard PC, et al.
Biomarkers, 20(8), 547-556 (2015)

Articoli

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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