172426

Glucoamylase, Rhizopus sp.

Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. Useful for the enzymatic determination of starch.

• Sinonimo/i: Glucoamylase, Rhizopus sp., Glucan 1,4-α-glucosidase, Glucoamylase, Amyloglucosidase, γ-Amylase, Lysosomal α-glucosidase, exo-4,7-α-glucosidase
• Numero CAS: 9032-08-0
• Classificazione EC (Enzyme Commission): 3.2.1.3 (BRENDA, IUBMB)
• Numero MDL: MFCD00081350
• Codice UNSPSC: 12352202

Proprietà

• Origine biologica: fungus (Rhizopus spp.)
• Livello qualitativo: 100
• Forma fisica: lyophilized
• Attività specifica: ≥10 unit/mg solid; ≥20 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Solubilità: aqueous buffer: 1 mg/mL; water: 1 mg/mL
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Native glucoamylase from Rhizopus species. Glucoamylase is a multi-domain enzyme that belongs to the glycosyl hydrolase 15 family. It catalyzes the hydrolysis of 1,4-linked α-D-glucose residues in glucosylpolysaccharides, liberating glucose units. Useful for the enzymatic determination of starch.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will liberate 1 µmol glucose from maltopentaose per min at 37°C, pH 6.0.

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Altre note

Hiromi, K., et al. 1973. Biochim. Biophys. Acta302, 362.
Kelly, J.J. and Alpers, D.H. 1973. Biochim. Biophys. Acta315, 113.
Jeffrey, P.L., et al. 1970. Biochemistry9, 1403.
Brown, B. and Brown, D.H. 1965. Biochim. Biophys. Acta110, 124.
Tsujisaka, Y., et al. 1958. Nature181, 770.
Miller, K.D. and Copeland, W.H. 1956. Biochim. Biophys. Acta22, 193.
French, D. and Knapp, D.W. 1950. J. Biol. Chem.187, 463.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable