05-858

Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

clone 62-141-13, Upstate^®, from rabbit

• Sinonimo/i: H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: 62-141-13, monoclonal
• Reattività contro le specie: chicken, Saccharomyces cerevisiae, vertebrates, yeast, human
• Produttore/marchio commerciale: Upstate^®
• tecniche: ChIP: suitable (ChIP-seq); multiplexing: suitable; western blot: suitable
• Isotipo: IgG
• N° accesso NCBI: NP_003529
• N° accesso UniProt: P62805
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: Saccharomyces cerevisiae ... Hhf1(852294) ; human ... H4C1(8359)

Descrizione

Descrizione generale

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

This antibody recognizes histone H4 independant of any post-translational modifications.

Specificità

Broad species cross-reactivity expected based on sequence homology.

Recognizes Histone H4, Mr 10 kDa.

Immunogeno

Epitope: a.a. 25-28

KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.

Applicazioni

Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.

Epitope Mapping (using Luminex^®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualità

Routinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)

Descrizione del bersaglio

10 kDa

Stato fisico

Format: Purified

Protein A purified

Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

Luminex is a registered trademark of Luminex Corp

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable