05-829-AF647

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 647 conjugate

clone DM1A, from mouse, ALEXA FLUOR^™ 647

• Sinonimo/i: Tubulin alpha-1 chain, α-Tubulin
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 647
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: DM1A, monoclonal
• Reattività contro le specie: guinea pig, rat, porcine, human, gerbil, mouse, bovine, avian
• tecniche: immunocytochemistry: suitable
• Isotipo: IgG1
• N° accesso NCBI: NP_001292201.1
• N° accesso UniProt: P02552
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... TUBA1A(7846)

Descrizione

Descrizione generale

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

Specificità

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.

Immunogeno

Epitope: C-terminal 25 amino acids.

Purified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

Applicazioni

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualità

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.

Descrizione del bersaglio

~50 kDa observed.

Stato fisico

Protein G Purified

Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable