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Documenti fondamentali

05-591

Sigma-Aldrich

Anti-Akt/PKB Antibody, PH Domain, clone SKB1

clone SKB1, Upstate®, from mouse

Sinonimo/i:

Anti-AKT, Anti-PKB, Anti-PKB-ALPHA, Anti-PRKBA, Anti-RAC, Anti-RAC-ALPHA

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About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

affinity purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

SKB1, monoclonal

Reattività contro le specie

rat, human, mouse

Confezionamento

antibody small pack of 25 μg

Produttore/marchio commerciale

Upstate®

tecniche

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

dry ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

mouse ... Akt1(11651)

Descrizione generale

Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Applicazioni

Detect Akt/PKB using this Anti-Akt/PKB Antibody, PH Domain, clone SKB1 validated for use in FC, IC, IP & WB.

Qualità

routinely evaluated by immunoblot on RIPA lysates from A431 cells

Descrizione del bersaglio

60 kDa

Stato fisico

Format: Purified
Protein G Purified immunoglobulin in Immunoaffinity Purified immunoglobulin in 0.02 M phosphate buffer, pH 7.6, 0.25M NaCl, 0.1% sodium azide and 30% glycerol.

Risultati analitici

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Note legali

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Evaluation of approaches to generation of tissue-specific knock-in mice.
Bayascas, JR; Sakamoto, K; Armit, L; Arthur, JS; Alessi, DR
The Journal of Biological Chemistry null
Regulation of AMP-activated protein kinase by multisite phosphorylation in response to agents that elevate cellular cAMP.
Hurley, RL; Barre, LK; Wood, SD; Anderson, KA; Kemp, BE; Means, AR; Witters, LA
The Journal of Biological Chemistry null
Important role of the LKB1-AMPK pathway in suppressing tumorigenesis in PTEN-deficient mice.
Huang X, Wullschleger S, Shpiro N, McGuire VA, Sakamoto K, Woods YL, McBurnie W et al.
The Biochemical Journal null
Heng-Liang Lin et al.
The Journal of pharmacology and experimental therapeutics, 323(1), 365-373 (2007-07-25)
Combretastatin A4 (CA4) is a drug that targets tumor vasculature to inhibit angiogenesis. Whether CA4 has a direct effect on gastric cancer is not known. We herein investigated the effect of CA4 on growth and metastasis of gastric cancer cells
HRT1 modulates vascular smooth muscle cell proliferation and apoptosis.
Wenli Wang, Chengyu Z Prince, Xing Hu, Matthew J Pollman
Biochemical and biophysical research communications null

Articoli

Autophagy is a highly regulated process that is involved in cell growth, development, and death. In autophagy cells destroy their own cytoplasmic components in a very systematic manner and recycle them.

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