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P5542

Sigma-Aldrich

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

Synonym(e):

PC-PLC

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About This Item

CAS-Nummer:
37288-19-0
EC-Nummer:
4.6.1.13 (BRENDA, IUBMB)
EG-Nummer:
232-638-2
MDL-Nummer:
MFCD00131896
UNSPSC-Code:
12352204
NACRES:
NA.32

Form

lyophilized powder

Qualitätsniveau

300

Spezifische Aktivität

≥1,000 units/mg protein (Lowry)

Fremdaktivität

Phopholipase C (lecithinase) ≤1 units/mg protein
Sphingomyelinase ≤40 units/mg protein

Lagertemp.

−20°C

Angaben zum Gen

Bacillus cereus E33L ... BCZK3513(3026815)

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Allgemeine Beschreibung

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

Anwendung

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

Biochem./physiol. Wirkung

Used for the release of GPI anchored proteins from the membrane.

Einheitendefinition

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Physikalische Form

Contains phosphate buffer salts, EDTA and stabilizer

Hinweis zur Analyse

Acetylcholinesterase is measured according to Ellman, et al.

Inhibitor

Produkt-Nr.
Beschreibung
Preisangaben

I2512

Natriumjodacetat, ≥98%

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Helen Neuert et al.
Development (Cambridge, England), 147(2) (2019-12-22)
The development of tissues and organs requires close interaction of cells. To achieve this, cells express adhesion proteins such as the neural cell adhesion molecule (NCAM) or its Drosophila ortholog Fasciclin 2 (Fas2). Both are members of the Ig-domain superfamily
Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane.
Brameshuber M
The Journal of Biological Chemistry, 285(53), 41765-41771 (2010)
Danielle K Bailey et al.
PloS one, 16(7), e0254794-e0254794 (2021-07-27)
Iron and other transition metals, such as copper and manganese, are essential for supporting brain function, yet over-accumulation is cytotoxic. This over-accumulation of metals, particularly iron, is common to several neurological disorders; these include Alzheimer's disease, Parkinson's disease, Friedrich's ataxia
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW
The Embo Journal, 14(16), 3855-3863 (1995)
Role of placental alkaline phosphatase in the internalization of trypomatigotes of Trypanosoma cruzi into HEp2 cells.
Sartori MJ
Tropical Medicine & International Health, 8(9), 832-839 (2003)
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Artikel

GPI Anchored Glycoproteins

GPI Anchored Glycoproteins

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