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P6621

Sigma-Aldrich

Phospholipase C aus Bacillus cereus

greener alternative

≥200 units/mg protein

Synonym(e):

Lecithinase C, Lipophosphodiesterase I, PC-PLC

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About This Item

CAS-Nummer:
9001-86-9
EC-Nummer:
3.1.4.3 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131895
UNSPSC-Code:
12352204
NACRES:
NA.54

Form

lyophilized powder

Qualitätsniveau

300

Spezifische Aktivität

≥200 units/mg protein

Mol-Gew.

23-27 kDa by SDS-PAGE

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Löslichkeit

H2O: soluble 1.0 mg/mL, faintly hazy to hazy

Grünere Alternativprodukt-Kategorie

Enabling,

Lagertemp.

−20°C

Angaben zum Gen

Bacillus cereus ATCC 10987 ... plC(2749087)

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Allgemeine Beschreibung

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.

Anwendung

Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling. It is used to degrade inositol-containing phospholipids. Phospholipase C from Bacillus, product P6621, has been used to study how flhF affects the export of PLC and other secretory virulence factors.
The enzyme from Sigma has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms.

Biochem./physiol. Wirkung

Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.

Einheitendefinition

One unit will liberate 1.0 μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.

Physikalische Form

Lyophilized powder containing approx. 10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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W Siess et al.
The Journal of biological chemistry, 259(13), 8286-8292 (1984-07-10)
The present study compares the molecular mechanism by which thrombin, platelet-activating factor, and epinephrine induce platelet activation. Thrombin and platelet-activating factor induce an initial activation of phospholipase C, as measured by formation of 1,2-diacylglycerol and phosphatidic acid, during platelet shape
Zollner, H.
Handbook of Enzyme Inhibitors, 387-387 (1993)
Sara Salvetti et al.
Microbiology (Reading, England), 153(Pt 8), 2541-2552 (2007-07-31)
Flagellar arrangement is a highly conserved feature within bacterial species. However, only a few genes regulating cell flagellation have been described in polar flagellate bacteria. This report demonstrates that the arrangement of flagella in the peritrichous flagellate Bacillus cereus is
T Das et al.
The Journal of biological chemistry, 268(36), 27398-27405 (1993-12-25)
Our studies on the alpha 1-adrenoceptor signaling have demonstrated that the Gh family is a signal mediator. We report here that a 69-kDa phospholipase C (PLC) is the effector in this signal pathway. The enzyme was isolated by dissociating a
Clarisse Techer et al.
Food microbiology, 86, 103317-103317 (2019-11-11)
The famous French dessert "ile flottante" consists of a sweet egg white foam floating on a vanilla custard cream, which contains highly nutritive raw materials, including milk, sugar and egg. Spoilage issues are therefore a key concern for the manufacturers.
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