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GE17-0980-01

Butyl Sepharose™ 4 Fast Flow

Cytiva 17-0980-01, pack of 200 mL

Synonym(e):

Butyl Sepharose, Fast Flow Sepharose

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

ligand

butyl

Verpackung

pack of 200 mL

Hersteller/Markenname

Cytiva 17-0980-01

Matrix

4% cross-linked agarose

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning in place

2-14

Arbeitsbereich

3-13

Eignung

suitable for bioprocess medium

Verwandte Kategorien

Allgemeine Beschreibung

Butyl Sepharose™ 4 Fast FLow is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification of larger proteins.

Anwendung

Butyl Sepharose™ 4 Fast FLow is part of the Sepharose™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Butyl Sepharose™ 4 Fast FLow is based on cross-linked 4% Agarose. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

Butyl Sepharose™ 4 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl Sepharose™ 4 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Leistungsmerkmale und Vorteile

  • Standard aliphatic HIC media based on Sepharose™ Fast FLow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Optimized for the separation of larger Proteins in capture and intermediate purification steps
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

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Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

Screening for Selectivity of HIC Medium

This section is about the different ways of screening for selectivity of HIC medium from GE Healthcare, considering both automated and manual

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

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Protokolle

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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