GE17-0973-05
Phenyl Sepharose™ 6 Fast Flow (High Sub)
Cytiva 17-0973-05, pack of 200 mL
About This Item
Empfohlene Produkte
ligand
phenyl
Verpackung
pack of 200 mL
Hersteller/Markenname
Cytiva 17-0973-05
Matrix
6% cross-linked agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning in place
2-14
working range
3-13
Eignung
suitable for bioprocess medium
Verwandte Kategorien
Allgemeine Beschreibung
Phenyl Sepharose™ 6 Fast Flow is part of the Sepharose™ Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Phenyl Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.
Phenyl Sepharose™ 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Phenyl Sepharose™ 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Leistungsmerkmale und Vorteile
- Standard aromatic HIC media based on Sepharose™ Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
- Designed for initial and intermediate purification requiring low to medium hydrophobicity
- Two levels of substitution help find optimal selectivity and binding capacity for a given application
- BioProcess medium supported for industrial applications and well-established in approved processes
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.
This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered
This page discusses column packing and preparation techniques for reverse phase chromatography
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
Protokolle
While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.
This page discusses how to do a purification with SOURCE™ media from GE Healthcare
This page discusses various aspects of sample preparation for chromatographic purification.
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