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GE17-1082-01

Phenyl Sepharose™ High Performance

Cytiva 17-1082-01, pack of 75 mL

Synonym(e):

Phenyl sepharose

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

ligand

phenyl

Verpackung

pack of 75 mL

Hersteller/Markenname

Cytiva 17-1082-01

Matrix

6% cross-linked agarose

Partikelgröße

24-44 μm

Durchschnittliche Teilgröße

34 μm

cleaning in place

2-14

working range

3-13

Eignung

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

Allgemeine Beschreibung

Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

Leistungsmerkmale und Vorteile

  • Aromatic HIC medium designed for intermediate purification and polishing steps
  • Selectivity similar to Phenyl Sepharose™ 6 Fast Flow (low sub) but higher resolution due to the smaller 34 μm bead size
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Screening for Selectivity of HIC Medium

This section is about the different ways of screening for selectivity of HIC medium from GE Healthcare, considering both automated and manual

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

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Protokolle

Selectivity and the Properties of a HIC Medium

While ligands contribute significantly to the degree of hydrophobicity of a medium, the matrix can also influence the final selectivity. Chromatography media for hydrophobic interaction are made from porous matrices, chosen for their physical stability, their chemical resistance to stringent cleaning conditions and their low level of non-specific interaction.

Sepharose High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose High Performance and we present some examples of media screening, capture and purification.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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