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GE17-0920-07

IMAC Sepharose High Performance

Cytiva 17-0920-07, pack of 100 mL

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

Verpackung

pack of 100 mL

Hersteller/Markenname

Cytiva 17-0920-07

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

working range

3-12

Kapazität

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Allgemeine Beschreibung

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Anwendung

Conveniently charge with your metal of choice. IMAC Sepharose FF is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2?, Zn2?, Ni2?, or Co2?); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose. Small bead size for high performance purification and high binding capacity.

Leistungsmerkmale und Vorteile

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
  • Conveniently charge with your metal of choice
  • 34 μm bead size for high performance purification
  • High binding capacity

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

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Protokolle

Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance

This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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