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GE17-5195-01

HiTrap® Phenyl High Performance

Cytiva 17-5195-01, pack of 5 × 5 mL

Synonym(e):

HiTrap Phenyl Column, Phenyl High Performance

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About This Item

UNSPSC-Code:
23151816
NACRES:
NA.56

ligand

phenyl

Verpackung

pack of 5 × 5 mL

Hersteller/Markenname

Cytiva 17-5195-01

Parameter

42 psi

Bettgröße

16 mm × 25 mm

Bettvolumen

5 mL

Säule ID

16 mm

Matrix

6% cross-linked agarose

Partikelgröße

24-44 μm

Durchschnittliche Teilgröße

34 μm

cleaning

2-14

Arbeitsbereich

3-13

Eignung

suitable for bioprocess medium (BioProcess medium.)
suitable for bioprocess medium

Allgemeine Beschreibung

HiTrap® Phenyl HP are prepacked Phenyl Sepharose™ High Performance columns for high-resolution hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.

Leistungsmerkmale und Vorteile

  • Provide convenient and fast small-scale protein purifications.
  • Phenyl Sepharose™ High Performance is a well-established aromatic HIC medium, designed for intermediate and polishing steps where high resolution has priority.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as AKTA design.
  • High flow rates and high capacities.
  • Excellent and easy scale-up achieved by connecting several HiTrap® columns in series or using HiPrep™ columns.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

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Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

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Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

Screening for Selectivity of HIC Medium

This section is about the different ways of screening for selectivity of HIC medium from GE Healthcare, considering both automated and manual

Salt Selection and Buffer Preparation

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

Salt Selection and Buffer Preparation

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

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Protokolle

Sepharose High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose High Performance and we present some examples of media screening, capture and purification.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

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