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G1270
L-Glutamine Synthetase from Escherichia coli
lyophilized powder, 400-2,000 units/mg protein
Synonym(e):
L-Glutamate:ammonia ligase (ADP-forming)
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.26
Empfohlene Produkte
Form
lyophilized powder
Qualitätsniveau
Spezifische Aktivität
400-2,000 units/mg protein
Aufgereinigt durch
affinity chromatography
Enthält
dithioerythritol as preservative
Zusammensetzung
Protein, ~5% Lowry
Löslichkeit
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
UniProt-Hinterlegungsnummer
Fremdaktivität
ATPase <0.2%
Lagertemp.
−20°C
Angaben zum Gen
Escherichia coli K12 ... glnA(948370)
Allgemeine Beschreibung
L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.
Anwendung
L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.
Biochem./physiol. Wirkung
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.
Degradative enzyme for glutamic acid
Einheitendefinition
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.
Physikalische Form
Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Structure-function relationships of glutamine synthetases
Eisenberg D, et al.
Biochimica et Biophysica Acta, Protein Structure and Molecular Enzymology, 1477(1-2), 122-145 (2000)
Reversible Adenylylation of Glutamine Synthetase Is Dynamically Counterbalanced during Steady-State
Okano H, et al.
Journal of molecular biology, 404(1), 522-536 (2010)
Ammonia acquisition in enteric bacteria: physiological role of the ammonium/methylammonium transport B (AmtB) protein
Soupene E, et al.
Proceedings of the National Academy of Sciences of the USA, 95(12), 7030-7034 (1998)
Structural Analysis of Glutamine Synthetase from Helicobacter pylori
Joo HK, et al.
Scientific Reports, 8(1), 11657-11657 (2018)
Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation
Jin DY, et al.
The Journal of Biological Chemistry, 272(49), 30952-30961 (1997)
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