G8880

Glutaminase aus E. coli

Grade V, lyophilized powder, 50-200 units/mg protein

• Synonym(e): L-Glutamine amidohydrolase
• CAS-Nummer: 9001-47-2
• EC-Nummer: 3.5.1.2 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131193
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Escherichia coli
• Qualitätsniveau: 200
• Typ: Grade V
• Form: lyophilized powder
• Spezifische Aktivität: 50-200 units/mg protein
• Mol-Gew.: 110 kDa
• Zusammensetzung: Protein, ~30% biuret
• Kationenspuren: NH₄⁺: <0.1 μg/unit
• Fremdaktivität: NADH Oxidase ≤0.01%
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

Anwendung

Glutaminase from E.coli has been used:

• for the determination of glutamine from the cerebral cortex tissue extract
• for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
• in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

Biochem./physiol. Wirkung

Glutaminase catalyzes the conversion of glutamine to glutamate.

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.

Einheitendefinition

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

Physikalische Form

Lyophilized powder containing potassium succinate and EDTA

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)