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A7437
Acetat-Kinase aus E. coli
lyophilized powder, ≥150 units/mg protein (biuret)
Synonym(e):
ATP:Acetatphosphotransferase
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Empfohlene Produkte
Form
lyophilized powder
Qualitätsniveau
Spezifische Aktivität
≥150 units/mg protein (biuret)
Zusammensetzung
Protein, 5.0-20.0%
UniProt-Hinterlegungsnummer
Fremdaktivität
adenosine triphosphatase and NADH oxidase ≤0.01%
Lagertemp.
−20°C
Angaben zum Gen
Escherichia coli K12 ... ackA(946775)
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Allgemeine Beschreibung
Acetate kinase belongs to acetate and sugar kinase/Hsc70/actin (ASKHA) structural superfamily. It exists as a dimer and has nucleotide binding site in the cleft between N and C-terminal domain.
Anwendung
Acetate Kinase from Escherichia coli has been used in assessing acetate kinase activity during the fermatation process of Gluconacetobacter xylinus and in kinetic modeling and steady state studies.
Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Escherichia coli has been used as part of an ATP-regenerating system to study the kinetics of agonist-stimulated transphosphatidylation .
Biochem./physiol. Wirkung
Beteiligt am Metabolismus von Propanoat, Pyruvat und Taurin
Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA . Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine.
Einheitendefinition
1 U entspricht der Enzymmenge, die 1 μmol Adenosin zu Inosin pro Minute bei pH 7.6 und 25°C deaminiert
Physikalische Form
Lyophilisiertes Pulver, enthält Trehalose mit wenig Kaliumphosphat, Magnesiumchlorid und Dithiothreitol
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
Buss KA, et al.
Journal of Bacteriology, 183(2), 680-686 (2001)
S C Olson et al.
The Journal of biological chemistry, 266(26), 17236-17242 (1991-09-15)
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F J Grundy et al.
Journal of bacteriology, 175(22), 7348-7355 (1993-11-01)
The Bacillus subtilis gene encoding acetate kinase was identified on the basis of sequence similarity to the Escherichia coli ackA gene and to a second E. coli gene closely related to ackA. Insertional inactivation of this region of the B.
Hélène Scornec et al.
Frontiers in microbiology, 11, 156-156 (2020-03-21)
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