H8629

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder

• Synonym(e): HK/G6PD
• MDL-Nummer: MFCD00131322
• UNSPSC-Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Eigenschaften

• Form: lyophilized powder
• Qualitätsniveau: 200
• glucose-6-phosphate dehydrogenase activity: ≥75 units/mg protein (biuret)
• hexokinase activity: ≥65 units/mg protein
• Aufgereinigt durch: crystallization
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Research area: Cell signaling

Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.

Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

Anwendung

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.

Biochem./physiol. Wirkung

Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.

Verpackung

Package sizes are based on hexokinase units.

Komponenten

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

Einheitendefinition

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)