C8571
Cathepsin B from human liver
buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)
Synonym(e):
Cathepsin B1
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352200
NACRES:
NA.32
Empfohlene Produkte
Biologische Quelle
human liver
Qualitätsniveau
Form
buffered aqueous solution
Spezifische Aktivität
≥1,500 units/mg protein (E1%/280)
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−70°C
Angaben zum Gen
human ... CTSB(1508)
Allgemeine Beschreibung
Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.
Anwendung
Cathepsin B from human liver has been used:
- as a positive control in the screening Cathepsin B inhibitor assay
- in wall extension (expansin activity) assay
- in microinjection human foreskin fibroblasts to test its effect on apoptosis induction
Biochem./physiol. Wirkung
Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.
Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.
Verpackung
Package size based on protein content
Einheitendefinition
One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.
Physikalische Form
Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.
Inhibitor
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Beschreibung
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Substrat
Ähnliches Produkt
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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Amino acid sequence of human liver cathepsin B
Ritonja A, et al.
Febs Letters, 181(1), 169-172 (1985)
Genome-wide catalogue of chromosomal aberrations in barrett's esophagus and esophageal adenocarcinoma: a high-density single nucleotide polymorphism array analysis
Gu J, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(9), 1176-1186 (2010)
The refined 2.15 AX-ray crystal structure of human liver cathepsin B: the structural basis for its specificity.
Musil DJ, et al.
The Embo Journal, 10(9), 2321-2330 (1991)
Cathepsin B inactivation attenuates hepatic injury and fibrosis during cholestasis
Canbay A, et al.
The Journal of Clinical Investigation, 112(2), 152-159 (2003)
Artikel
Enzymatic Assay of Cathepsin B
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