C3138
Kathepsin D aus Rindermilz
lyophilized powder, ≥2.0 units/mg protein
Synonym(e):
CTSD
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About This Item
CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.32
Empfohlene Produkte
Biologische Quelle
bovine spleen
Assay
10—70% protein (biuret)
Form
lyophilized powder
Spezifische Aktivität
≥2.0 units/mg protein
Mol-Gew.
~45 kDa
Hersteller/Markenname
Sigma-Aldrich
Methode(n)
activity assay: suitable
Farbe
dark brown
Eignung
suitable for molecular biology
UniProt-Hinterlegungsnummer
Anwendung(en)
life science and biopharma
Lagertemp.
−20°C
Angaben zum Gen
cow ... CTSD(282883)
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Allgemeine Beschreibung
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Anwendung
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
Biochem./physiol. Wirkung
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Einheitendefinition
One unit will produce an increase in A280 of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).
Physikalische Form
Lyophilized powder containing citrate buffer salts
Inhibitor
Produkt-Nr.
Beschreibung
Preisangaben
Ähnliches Produkt
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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