C3138

Kathepsin D aus Rindermilz

lyophilized powder, ≥2.0 units/mg protein

• Synonym(e): CTSD
• CAS-Nummer: 9025-26-7
• EC-Nummer: 3.4.23.5 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130749
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: bovine spleen
• Assay: 10—70% protein (biuret)
• Form: lyophilized powder
• Spezifische Aktivität: ≥2.0 units/mg protein
• Mol-Gew.: ~45 kDa
• Hersteller/Markenname: Sigma-Aldrich
• Methode(n): activity assay: suitable
• Farbe: dark brown
• Eignung: suitable for molecular biology
• UniProt-Hinterlegungsnummer: P80209
• Anwendung(en): life science and biopharma
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... CTSD(282883)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.

Anwendung

Cathepsin D from bovine spleen has been used:

• in in vitro dose-dependent fluorometric activity assays.
• in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
• for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
• in cathepsin D activity assay.

Biochem./physiol. Wirkung

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Einheitendefinition

One unit will produce an increase in A₂₈₀ of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).

Physikalische Form

Lyophilized powder containing citrate buffer salts

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable