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Key Documents

SRP0293

Sigma-Aldrich

Cathepsin V Active human

recombinant, expressed in FreeStyle™ 293-F cells, ≥90% (SDS-PAGE)

Synonym(e):

Cathepsin L2, Cathepsin U

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About This Item

UNSPSC-Code:
12352204
NACRES:
NA.54

Rekombinant

expressed in FreeStyle™ 293-F cells

Assay

≥90% (SDS-PAGE)

Form

aqueous solution

Spezifische Aktivität

≥436 pmol/min-μg

Mol-Gew.

37 kDa

NCBI-Hinterlegungsnummer

NM_001333.2

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... CTSL2(1515)

Allgemeine Beschreibung

Human cathepsin V (GenBank Accession No. NM_001333.2), CD33 signal peptide(amino acids 1-16) + cathepsin V (amino acids 18-334), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

Anwendung

Active human cathepsin V is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin V has been used in a study to assess the cysteine protease attribute of the eukaryotic ribosomal protein S4. Active human cathepsin V has also been used in a study to determine that the simultaneous treatment of human bronchial epithelial cells with serine and cysteine protease inhibitors prevents severe acute respiratory syndrome coronavirus entry.

Einheitendefinition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physikalische Form

Formulated in 40 mM MES, pH 5.0, 120 mM NaCL and 20% glycerol.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Rechtliche Hinweise

FreeStyle is a trademark of Invitrogen Corp.

Piktogramme

Exclamation markHealth hazard
GHS07,GHS08

Signalwort

Danger

Gefahreneinstufungen

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Lagerklassenschlüssel

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Babu Sudhamalla et al.
Biochimica et biophysica acta, 1820(10), 1535-1542 (2012-05-15)
Ribosomal proteins often carry out extraribosomal functions. The protein S4 from the smaller subunit of Escherichia coli, for instance, regulates self synthesis and acts as a transcription factor. In humans, S4 might be involved in Turner syndrome. Recent studies also
Miyuki Kawase et al.
Journal of virology, 86(12), 6537-6545 (2012-04-13)
The type II transmembrane protease TMPRSS2 activates the spike (S) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) on the cell surface following receptor binding during viral entry into cells. In the absence of TMPRSS2, SARS-CoV achieves cell entry via
Uta Schurigt et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 61(9), 1021-1029 (2012-06-08)
Cathepsin L (CL) is potentially involved in joint destruction and in antigen presentation in rheumatoid arthritis. In order to define the roles of this protease in arthritis development we analysed the antigen-induced arthritis (AIA) in CL-deficient (CL(-/-)) mice. Antigen-induced arthritis
Ryan P McGlinchey et al.
Biochemical and biophysical research communications, 529(4), 1106-1111 (2020-08-21)
The intracellular accumulation of α-synuclein (α-syn) amyloid fibrils is a hallmark of Parkinson's disease. Because lysosomes are responsible for degrading aggregated species, enhancing lysosomal function could alleviate the overburden of α-syn. Previously, we showed that cysteine cathepsins (Cts) is the

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