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46985

Sigma-Aldrich

Fluoreszein-5-thiosemicarbazid

suitable for fluorescence, ~80% (HPCE)

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About This Item

Empirische Formel (Hill-System):
C21H15N3O5S
CAS-Nummer:
76863-28-0
Molekulargewicht:
421.43
MDL-Nummer:
MFCD00155658
UNSPSC-Code:
12352125
PubChem Substanz-ID:
329757284
NACRES:
NA.32

Assay

~80% (HPCE)

Qualitätsniveau

100

Form

solid

Löslichkeit

DMF: soluble
H2O: soluble

Fluoreszenz

λex 492 nm; λem 516 nm in 0.1 M Tris pH 9.0

Eignung

suitable for fluorescence

Lagertemp.

−20°C

SMILES String

NNC(=S)Nc1ccc(c(c1)C(O)=O)C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24

InChI

1S/C21H15N3O5S/c22-24-21(30)23-10-1-4-13(16(7-10)20(27)28)19-14-5-2-11(25)8-17(14)29-18-9-12(26)3-6-15(18)19/h1-9,25H,22H2,(H,27,28)(H2,23,24,30)

InChIKey

MEUCHQDLZYLNQY-UHFFFAOYSA-N

Verwandte Kategorien

Allgemeine Beschreibung

Fluorescein-5-thiosemicarbazide, also known as FTC, is a fluorescent probe. The FTSC fluorescent spectra is like FITC, a preferred fluorescent tag for proteomics. The fluorescence spectral properties of FTSC-labeled vesicles obtained by reductive amination are fully compatible with the argon laser of fluorescent instruments.

Anwendung

Markierung von Funktionellen Gruppen an der Zelloberfläche (Glycophorine)

Leistungsmerkmale und Vorteile

Fluorescein-5-thiosemicarbazide has the following benefits -

  • It displays strong fluorescence property.
  • Shows an intrinsic reactivity of the thiosemicarbazide group towards the aldehyde group.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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One-pot fluorescent labeling of saccharides with fluorescein-5-thiosemicarbazide for imaging polysaccharides transported in living cells
Zhang Y, et al.
Carbohydrate Research, 346(14), 2156-2164 (2011)
Shinichi Nishimura et al.
PloS one, 8(12), e83716-e83716 (2014-01-05)
Cholesterol plays important roles in biological membranes. The cellular location where cholesterol molecules work is prerequisite information for understanding their dynamic action. Bioimaging probes for cholesterol molecules would be the most powerful means for unraveling the complex nature of lipid
Carina C Clingman et al.
eLife, 3 (2014-06-18)
Gene expression and metabolism are coupled at numerous levels. Cells must sense and respond to nutrients in their environment, and specialized cells must synthesize metabolic products required for their function. Pluripotent stem cells have the ability to differentiate into a
J D Corbett et al.
Biophysical journal, 66(1), 25-30 (1994-01-01)
We have used the fluorescence photobleaching recovery technique to study the dependence on oxygen tension of the lateral mobility of fluorescently labeled band 3, the phospholipid analogue fluorescein phosphatidylethanolamine, and glycophorins in normal red blood cell membranes. Band 3 protein
Hua Tian et al.
Journal of cellular and molecular medicine, 21(1), 107-120 (2016-08-12)
Oxidative stress and inflammation play important roles in the pathogenesis of cardiovascular disease (CVD). Oxidative stress-induced desialylation is considered to be a primary step in atherogenic modification, and therefore, the attenuation of oxidative stress and/or inflammatory reactions may ameliorate CVD.
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