46950

Fluoreszein-5(6)-isothiocyanat

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

• Synonym(e): FITC
• Empirische Formel (Hill-System): C₂₁H₁₁NO₅S
• CAS-Nummer: 27072-45-3
• Molekulargewicht: 389.38
• Beilstein: 1407295
• EG-Nummer: 248-207-7
• MDL-Nummer: MFCD00005063
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 329757273
• NACRES: NA.32

Eigenschaften

• Produktlinie: BioReagent
• Assay: ≥90% (HPLC)
• Fluoreszenz: λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
• Eignung: suitable for fluorescence
• Lagertemp.: −20°C
• SMILES String: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChIKey: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Anwendung

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.

Für die Fluoreszenzmarkierung von Proteinen (z.B. Immunofluoreszenz); H Protein-Labeling für die Gelchromatographie.

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves