GE28-9365-51
Hisprep™ Fast Flow 16/10
Cytiva 28-9365-51
Synonyme(s) :
16/10 Hisprep, Hisprep Column, Hisprep Fast Flow Column
About This Item
Produits recommandés
Conditionnement
pkg of 1 ea
Fabricant/nom de marque
Cytiva 28-9365-51
Paramètres
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
Taille du lit
16 mm × 100 mm
Volume du lit
20 mL
D.I. de la colonne
16 mm
Matrice
6% cross-linked agarose
Taille des particules
45-165 μm
Diamètre moyen
90 μm
cleaning
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
Capacité
~40 mg binding capacity(histidine-tagged protein)
Adéquation
suitable for bioprocess medium
Catégories apparentées
Description générale
HisPrep™ FF 16/10 columns are prepacked with Ni Sepharose™ 6 Fast Flow for scale-up purification of histidine-tagged proteins. Ni Sepharose™ 6 Fast Flow consists of 90 ?m beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.
Caractéristiques et avantages
- Optimized for convenient scale-up purification of histidine-tagged proteins.
- Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
- Negligible leakage of Ni2+.
- High binding capacity, approx. 40 mg/mL medium.
- Convenient and time-saving 20 mL prepacked HiPrep™ format.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
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